| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189394 |
| Chromosome: | chromosome 13 |
| Location: | 4954086 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606300 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAGGCGGGCTGTGTTATGTTTTAGATG |
| Internal bar code: | CTGGGAACTGGGGTAGGTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.8 |
| LEAP-Seq n confirming: | 1497 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCACTCAAACGCTAAGA |
| Suggested primer 2: | CAAACCATTGAATTGGGGAG |