| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189397 |
| Chromosome: | chromosome 10 |
| Location: | 2531252 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436600 | RSGA1,RSGA | Putative methyltransferase involved in ribosome biogenesis; (1 of 1) K06949 - ribosome biogenesis GTPase [EC:3.6.1.-] (rsgA, engC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCGGGACACAGACGCCGCACCGGCGGC |
| Internal bar code: | AAGCATGGTCTGGCAGGGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCCTAGCTTTGGAAACG |
| Suggested primer 2: | TCAGCACCACAGGCTCATAG |