| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189436 |
| Chromosome: | chromosome 7 |
| Location: | 2426102 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328850 | CNK8 | NimA-related protein kinase; (1 of 1) PTHR24361:SF240 - PROTEIN MTK-1, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGCATGACTCACAAGCCCCGTCGCTAA |
| Internal bar code: | GCGCATATCGGACAATCTGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 28 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGTGCTTGAAGAACTG |
| Suggested primer 2: | TTTTGAGTGTCTCGGAGGCT |