| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189463 |
| Chromosome: | chromosome 2 |
| Location: | 1575970 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084950 | GUK1 | Guanylate kinase; (1 of 1) K00942 - guanylate kinase (E2.7.4.8, gmk) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACTGACGCGATCCCTTGCCTCTCCAC |
| Internal bar code: | GGATCGGATCCTAACTCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.8554 |
| LEAP-Seq n confirming: | 2072 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTCTTAGGAGCATCAG |
| Suggested primer 2: | GGTATGAGAGTGGCCGTGAT |