Insertion junction: LMJ.RY0402.189509_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.189509
Chromosome:chromosome 6
Location:2352759
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g267500 (1 of 1) K02735 - 20S proteasome subunit beta 3 (PSMB3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCCCACAGGCAACCCCACCCCAACCAC
Internal bar code:GTACAACCAAGTTCACTGACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:37
LEAP-Seq percent confirming:44.012
LEAP-Seq n confirming:147
LEAP-Seq n nonconfirming:187
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACCACCTTGTCCTTGGT
Suggested primer 2:GTGTTCCTGCACACATGGTC