| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189539 |
| Chromosome: | chromosome 5 |
| Location: | 1880460 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAATACCTACCACTCTGTCACCTTTGAC |
| Internal bar code: | AAAGCAGTAGTGCCGGTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 98.4656 |
| LEAP-Seq n confirming: | 1476 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTGGCACCTATCCCTTG |
| Suggested primer 2: | CGTGAGCTTCGGCTTAAATC |