| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189543 |
| Chromosome: | chromosome 6 |
| Location: | 3004980 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273650 | (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGGTTGCATAGTACGCGACCCTCTCT |
| Internal bar code: | TCGTAACCTCTCAGTCAGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 98.6301 |
| LEAP-Seq n confirming: | 216 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGGGGTCTTCTTGTGT |
| Suggested primer 2: | GCCATCCACCTCAAGAACAT |