Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.189551 |
Chromosome: | chromosome 9 |
Location: | 7497249 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g413900 | GT90F18,GT90-18 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 18 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCACATAACGTAAGCATACACACTTCTT |
Internal bar code: | ACGTCGTAAGCGGGCGAGGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 796 |
LEAP-Seq percent confirming: | 97.3051 |
LEAP-Seq n confirming: | 1011 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTATGATGCGCTCGATTTGA |
Suggested primer 2: | AGTCGAATCATTACGTCGGC |