| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189580 |
| Chromosome: | chromosome 6 |
| Location: | 2994364 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273450 | POB11 | Proteome of basal body 11 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAAGGCACGTGGGGGAGGGGGGAGAG |
| Internal bar code: | TGATGTGGCTGCTTGATGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 11 |
| LEAP-Seq percent confirming: | 99.0521 |
| LEAP-Seq n confirming: | 418 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGGCTAGGACTCACTG |
| Suggested primer 2: | TGAGGACTTTGGGAAACTGG |