| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189580 |
| Chromosome: | chromosome 9 |
| Location: | 6735381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409000 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCGCATCCTTGATGGGCGCGTTGTTG |
| Internal bar code: | GGTAGATCCCTGGCCGTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 93.6117 |
| LEAP-Seq n confirming: | 1861 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTACCTTTGGGAACGG |
| Suggested primer 2: | ACCACACCTAACCCTTGTGC |