| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189582 |
| Chromosome: | chromosome 17 |
| Location: | 3843223 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTTTGCCCTCACCGCTGCAACGGTCGC |
| Internal bar code: | TCTACGGCCGCTACCCCGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 99.8033 |
| LEAP-Seq n confirming: | 1015 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATGAGGGACCCTTCCACC |
| Suggested primer 2: | TAGGAGGGTGGTGACCTGAC |