| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189583 |
| Chromosome: | chromosome 8 |
| Location: | 1482621 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364751 | BLZ16 | bZIP transcription factor | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCTCCAGGGAGAAGTCGAATTTCGTAA |
| Internal bar code: | CTCACTGTGGCAAACTCTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 96.4637 |
| LEAP-Seq n confirming: | 982 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACGGTGTGGTATGACCC |
| Suggested primer 2: | AGGGAGCTCGTTTGATGCTA |