| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189586 |
| Chromosome: | chromosome 1 |
| Location: | 4515314 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGCAATTTCGCAAGCATGACAATGAC |
| Internal bar code: | AAACCGGATGGAGACACGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 99.3772 |
| LEAP-Seq n confirming: | 20744 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGCTTTGGATTTCCTCA |
| Suggested primer 2: | TGACGTTGTGGTGACTCCAT |