| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189606 |
| Chromosome: | chromosome 8 |
| Location: | 3882028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377950 | (1 of 3) PF02622 - Uncharacterized ACR, COG1678 (DUF179) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGGGCTCATCGCGTGAACGCTTCCTGC |
| Internal bar code: | GCCCTGGATAGCCTTTCCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 97.4281 |
| LEAP-Seq n confirming: | 1288 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCCTCCTTTTGTCTCG |
| Suggested primer 2: | AGGATCGAAGGGAAACTGCT |