| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189616 |
| Chromosome: | chromosome 14 |
| Location: | 1915647 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620951 | (1 of 1) PTHR21496//PTHR21496:SF8 - FERREDOXIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCCAGGCATGACTTTTAAGACGGCGGT |
| Internal bar code: | CTGGGTTAGGGGATTGGCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.4755 |
| LEAP-Seq n confirming: | 1138 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACTAGCCTCCAACCACC |
| Suggested primer 2: | GCGATGAGTAACGCAAGTGA |