| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189672 |
| Chromosome: | chromosome 7 |
| Location: | 2612148 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330200 | PF17,RSP9 | Radial Spoke Protein 9 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAACGGCAAGGTAGGGGTGTGCCCAGCA |
| Internal bar code: | AATGGGCTACTCTTGGTGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 99.8959 |
| LEAP-Seq n confirming: | 1920 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTCTCTCAACCCGATA |
| Suggested primer 2: | ATGCTATTCGGTTTGTTGCC |