Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.189672 |
Chromosome: | chromosome_7 |
Location: | 2612148 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g330200 | RSP9 | Radial spoke protein 9 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGAACGGCAAGGTAGGGGTGTGCCCAGCA |
Internal bar code: | AATGGGCTACTCTTGGTGCCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 114 |
LEAP-Seq percent confirming: | 99.8959 |
LEAP-Seq n confirming: | 1920 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGGGTCTCTCAACCCGATA |
Suggested primer 2: | ATGCTATTCGGTTTGTTGCC |