| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189674 |
| Chromosome: | chromosome_4 |
| Location: | 3418590 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g227600 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTATCGGTGCATGCGTTTGTGGTACCCT |
| Internal bar code: | CTCGATCGTCTACACCTACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.3978 |
| LEAP-Seq n confirming: | 5612 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTACGGTTGTACGGCT |
| Suggested primer 2: | CTCTAAGGACTGGAGCGGTG |