| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189685 |
| Chromosome: | chromosome 16 |
| Location: | 6934994 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676981 | (1 of 8) IPR008962 - PapD-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAGGTGAGATGGCGTCCGCGCGGGCCG |
| Internal bar code: | TCCGTGAGGCCGGGCTCCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 191 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTTGTCTCTCGCAGCTT |
| Suggested primer 2: | GGTAGGGTACAGGGGATGGT |