Insertion junction: LMJ.RY0402.189691_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.189691
Chromosome:chromosome 3
Location:7653545
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g204100 ELG5 Exostosin-like glycosyltransferase 5; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCACGCCACCGGAAGGCTCGCAACCCTC
Internal bar code:GAGACTGATCGGAGATGGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:99.8759
LEAP-Seq n confirming:805
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTATGCGACTTGACGAGGTG
Suggested primer 2:GGACACATATGCAAACACGC