| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189695 |
| Chromosome: | chromosome 10 |
| Location: | 4233194 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450100 | (1 of 1) K04957 - hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCGGGCGGTATCTGGGGCGCACCAGA |
| Internal bar code: | GTCCTGTTATTGCTGTAGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 99.6946 |
| LEAP-Seq n confirming: | 5223 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATACACGGGTTGAGGCGT |
| Suggested primer 2: | CAGAGGCTGATGAAGCACAA |