| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189697 |
| Chromosome: | chromosome 1 |
| Location: | 3731933 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023950 | (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTCGGCCCGCTCGCCGCTGTCTGCCCC |
| Internal bar code: | ACGAAGAAGGGACGATACTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 89.5294 |
| LEAP-Seq n confirming: | 1522 |
| LEAP-Seq n nonconfirming: | 178 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGTCATACAGCCACAGC |
| Suggested primer 2: | GAGGGACAGAACATGGAGGA |