Insertion junction: LMJ.RY0402.189699_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.189699
Chromosome:chromosome 3
Location:4523607
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g176500 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAAGAAATGTAAGCTAGTGTGAATGCTA
Internal bar code:TCATCGACGCAAGGGTTGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1097
LEAP-Seq percent confirming:86.3072
LEAP-Seq n confirming:31421
LEAP-Seq n nonconfirming:4985
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGAGTGATGATGGCACA
Suggested primer 2:TGTTTACTTGCTCGTGGTCG