| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189699 |
| Chromosome: | chromosome 3 |
| Location: | 5637578 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187300 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTATTAAGCATGTCCTTTTACGAGCAA |
| Internal bar code: | TACGCCTATGCGACTAGTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 58.8562 |
| LEAP-Seq n confirming: | 1060 |
| LEAP-Seq n nonconfirming: | 741 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACCAGCAGCCAAACACC |
| Suggested primer 2: | ATGTACTCTCTGGGCGTGCT |