| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189717 |
| Chromosome: | chromosome 7 |
| Location: | 434556 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315300 | CSG4 | Chlamydomonas specific family G protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCAAGCTGGTGCTAGAGGGCCAGAGC |
| Internal bar code: | TCCCCTGAAGTAGGACCCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2729 |
| LEAP-Seq percent confirming: | 94.1667 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAAGGAGGCTGAGGTGA |
| Suggested primer 2: | TGTTCAGCGTCTGTAGGTGC |