| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189722 |
| Chromosome: | chromosome 6 |
| Location: | 8356625 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306550 | (1 of 1) K01166 - ribonuclease T2 [EC:3.1.27.1] (E3.1.27.1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTAAAGACTGGTTGAAGGAGGGCGTGGCG |
| Internal bar code: | AAGGGGGGGCGGTGAAAGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 99.4378 |
| LEAP-Seq n confirming: | 2653 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTTGCCTGGCGTTACTA |
| Suggested primer 2: | GCGTGTGATGGGACAGTATG |