| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189741 |
| Chromosome: | chromosome 4 |
| Location: | 1468083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214000 | (1 of 1) PTHR19331//PTHR27000 - LYSYL OXIDASE-RELATED // FAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTGACTTGCACAATCGACAAATATAC |
| Internal bar code: | CATCGGCGGGTCGAAGCAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCAAGTGCCAGCAGAAA |
| Suggested primer 2: | TTCCTCGGCTTGCACTATCT |