| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189743 |
| Chromosome: | chromosome 14 |
| Location: | 2205446 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622800 | (1 of 1) IPR000104//IPR002921//IPR029058 - Antifreeze protein, type I // Fungal lipase-like domain // Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGCGGCAGCAGCTTTACGGCCCGATAA |
| Internal bar code: | CCGCCCCGTGGTGGGGGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCGTCCCTACACACAC |
| Suggested primer 2: | AGGCACATGCGTACAGTCAG |