Insertion junction: LMJ.RY0402.189777_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.189777
Chromosome:chromosome_11
Location:2080245
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre11.g469000 FAP107 Flagellar Associated Protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TAGGCCCCGGGTACTCCAACTGGCCAGCAC
Internal bar code:TTTATTCAAGACTGTTGTTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:844
LEAP-Seq percent confirming:99.5658
LEAP-Seq n confirming:4586
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGCGCTACTACGGAAATA
Suggested primer 2:TCGTTGCTAGGCAACATCAG