| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189780 |
| Chromosome: | chromosome 13 |
| Location: | 4556506 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603750 | KCA1 | (1 of 2) PTHR10027 - CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN; Six-transmembrane domain potassium channel alpha subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACCCAAGTGGGGCAGGGTGCTGCGGGC |
| Internal bar code: | ACTAGGTTCTATAAGCTCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 99.4949 |
| LEAP-Seq n confirming: | 394 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGCAGTTGCATTGGTA |
| Suggested primer 2: | AGGACCTGGACGTGTTGTTC |