Insertion junction: LMJ.RY0402.189809_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.189809
Chromosome:chromosome 1
Location:5704460
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g040600 FAP327 Flagellar Associated Protein 327; (1 of 3) PTHR18937//PTHR18937:SF254 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGTTGCGGAATGCTGTTACGGTTCCG
Internal bar code:AGATGAGGTCGAAATCGGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:99.6821
LEAP-Seq n confirming:2195
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGGTGATAGCTCCACCGT
Suggested primer 2:TCGACCTACAACCTGGTTCC