| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189840 |
| Chromosome: | chromosome 12 |
| Location: | 9606712 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549852 | (1 of 14) IPR002198//IPR002347 - Short-chain dehydrogenase/reductase SDR // Glucose/ribitol dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCATTCACCCTGGCCCGTAAGAAACA |
| Internal bar code: | TATTCCGTATCGTACGCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 99.8377 |
| LEAP-Seq n confirming: | 615 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATACATAAACACGCACG |
| Suggested primer 2: | ACTTGCAGTTGATTGGGTCC |