Insertion junction: LMJ.RY0402.189857_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.189857
Chromosome:chromosome 7
Location:590919
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g316650 (1 of 1) PF00069//PF03924//PF07714 - Protein kinase domain (Pkinase) // CHASE domain (CHASE) // Protein tyrosine kinase (Pkinase_Tyr) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAAGTCCCGACGACTCTGGTATGGTCGC
Internal bar code:GGGCCGGGGCATTAGTGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1059
LEAP-Seq percent confirming:99.7704
LEAP-Seq n confirming:3042
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTCATCTGCTCGGTTCTG
Suggested primer 2:GGAGACGTCTTCTCACCGAG