| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189868 |
| Chromosome: | chromosome 2 |
| Location: | 1980142 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088250 | MCT2 | Malonyl-CoA:acyl-carrier-protein transacylase; (1 of 2) K00645 - [acyl-carrier-protein] S-malonyltransferase [EC:2.3.1.39] (fabD) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACGCAAAGCGCAGCCGCGGTCACGATC |
| Internal bar code: | CTTGTCGTGAGATCTGCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.2883 |
| LEAP-Seq n confirming: | 279 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGGTCACAAAGTAGGGA |
| Suggested primer 2: | CTTGTCCCTTCTTGCTACGC |