| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189897 |
| Chromosome: | chromosome 17 |
| Location: | 4415217 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731700 | FMO2 | Flavin-containing monooxygenase; (1 of 1) PF00743//PF13450 - Flavin-binding monooxygenase-like (FMO-like) // NAD(P)-binding Rossmann-like domain (NAD_binding_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATATGCCGCAACGTGGGCTCGAGCCCA |
| Internal bar code: | TCTCGTTTCTTTTGTTCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 99.2266 |
| LEAP-Seq n confirming: | 2566 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTCTCATCGTGTCTCA |
| Suggested primer 2: | CTGCTGCGTTAGGGAAGAAC |