| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189952 |
| Chromosome: | chromosome 14 |
| Location: | 3979491 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633300 | (1 of 1) IPR003582//IPR006626//IPR009030 - ShKT domain // Parallel beta-helix repeat // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCCCGCCAGCGCGCCCGCCCGCCTGCC |
| Internal bar code: | TACGCCTAGCGGAGCATCAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 90.7801 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGTTAGTTGGCGGTTG |
| Suggested primer 2: | AATAATGATCCGTTGCCAGC |