| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189962 |
| Chromosome: | chromosome 13 |
| Location: | 4171879 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592100 | (1 of 1) PF00097//PF00628 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4) // PHD-finger (PHD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATTCCCCGCTCGCTGCTCATCTCCCCT |
| Internal bar code: | TCTAACTTCCCCCTTCCGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 98.1651 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTGTGGTAGCCTCGGTC |
| Suggested primer 2: | AGGGCTACGTGCAATCATTC |