| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189970 |
| Chromosome: | chromosome 3 |
| Location: | 3583880 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168300 | (1 of 1) 3.4.21.107//3.4.21.108 - Peptidase Do / Protease Do // HtrA2 peptidase / Serine proteinase OMI | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTATTGTCTGGCCGCATCAAGCTAAACAA |
| Internal bar code: | AATGACGGGGAGAGGTGCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 968 |
| LEAP-Seq percent confirming: | 98.5014 |
| LEAP-Seq n confirming: | 1709 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCATTAACGTTTGCTGA |
| Suggested primer 2: | AATGCAGTACGTCAGTCCCC |