| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189983 |
| Chromosome: | chromosome 1 |
| Location: | 6234137 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044600 | (1 of 2) PF17047 - Synaptotagmin-like mitochondrial-lipid-binding domain (SMP_LBD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGTGCACCCCAGGCGTGCCCTCAGGT |
| Internal bar code: | ACTGTACTCACGGGTGGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1148 |
| LEAP-Seq percent confirming: | 99.4864 |
| LEAP-Seq n confirming: | 1937 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTCTTGCGACTCCTACG |
| Suggested primer 2: | TGCAGAGATCGCACGATTAG |