Insertion junction: LMJ.RY0402.190041_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.190041
Chromosome:chromosome 14
Location:490762
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g611250 POC9 Proteome of centriole protein 9; (1 of 2) PTHR12086:SF9 - EF-HAND DOMAIN-CONTAINING PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGGTGGTGATGGTGGCGGATGGTGATG
Internal bar code:CAGTGAGCGATCATCTGATAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:41
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGGATCAGTACTACGGTGCG
Suggested primer 2:CATCGTGCCTGTCGCTAGTA