Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.190045 |
Chromosome: | chromosome_16 |
Location: | 4482679 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g667450 | TLP2 | Tubby-like protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCATGCTGCCGCTGATGTGGCCGCCGGTG |
Internal bar code: | GATCGTTTGGCTAAAGGTACCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 685 |
LEAP-Seq percent confirming: | 59.5012 |
LEAP-Seq n confirming: | 501 |
LEAP-Seq n nonconfirming: | 341 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAACAATGCCTTTCACACCC |
Suggested primer 2: | GGAAATCTCTGCGTTTGAGC |