| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190066 |
| Chromosome: | chromosome 6 |
| Location: | 1648545 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260950 | (1 of 1) K12483 - EH domain-containing protein 1 (EHD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGAGTCAACCCCCCTCCACGCACCTC |
| Internal bar code: | ACCCCGTTATGTCAAACATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 99.546 |
| LEAP-Seq n confirming: | 5481 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTAGCGGCAAGGGTAATG |
| Suggested primer 2: | TTCTCTTTCTGCGGTGGACT |