| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190066 |
| Chromosome: | chromosome 6 |
| Location: | 1648547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260950 | (1 of 1) K12483 - EH domain-containing protein 1 (EHD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAATCCCGTAAGGAAACCGGGGAGGGGG |
| Internal bar code: | AGTAGAGTGTGCATAACGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 358 |
| LEAP-Seq percent confirming: | 99.6064 |
| LEAP-Seq n confirming: | 2784 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTAGCGGCAAGGGTAATG |
| Suggested primer 2: | TTCTCTTTCTGCGGTGGACT |