| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190072 |
| Chromosome: | chromosome 10 |
| Location: | 2404917 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435450 | CPL2 | (1 of 9) PTHR10071 - TRANSCRIPTION FACTOR GATA GATA BINDING FACTOR; transcription factor-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGGCCCCCGCAGCCCCGCTCCCATTTC |
| Internal bar code: | CGTAGGACCTAGAATGTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.2556 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGATGCATGAGGAACGAT |
| Suggested primer 2: | AAAGTGTCCAGGGACCACAG |