| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190073 |
| Chromosome: | chromosome 3 |
| Location: | 1946595 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155550 | DNJ18 | (1 of 1) IPR001623//IPR012336 - DnaJ domain // Thioredoxin-like fold; DnaJ-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCAACCCCGGCAGCAGCAACAGCAGC |
| Internal bar code: | GGGGGGTACCGCCAAATAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 99.6956 |
| LEAP-Seq n confirming: | 655 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCATCGTCTGCTTTCAT |
| Suggested primer 2: | GTGCTGTTATTTGAAGCGCA |