| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190130 |
| Chromosome: | chromosome 16 |
| Location: | 3574553 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681301 | MOT12 | Predicted protein; (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACTGGCGTAAATCACCGCACGGCATGC |
| Internal bar code: | GCCGCCGTACCAGGGGGGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 97.1963 |
| LEAP-Seq n confirming: | 1560 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGCGTTTGTTCGGGTAT |
| Suggested primer 2: | ACTGTTGAACTGGTCGTCCC |