| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190138 |
| Chromosome: | chromosome 17 |
| Location: | 1833086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709800 | RLS1 | RegA/Rls-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTAGCCGCTATGCCCCGATGCACAGTGT |
| Internal bar code: | TCGGCAATAAGGCACAGTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 98.7487 |
| LEAP-Seq n confirming: | 1973 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGTGATTACGGAGTGGC |
| Suggested primer 2: | TCTTTGCCTGGTCTCGAGTT |