Insertion junction: LMJ.RY0402.190150_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.190150
Chromosome:chromosome 13
Location:3741342
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAAACACAACTCGCTTTCACTCCAACTT
Internal bar code:TTGATCTACATCAGAGGGGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:719
LEAP-Seq percent confirming:99.7909
LEAP-Seq n confirming:1432
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGCCTGGTTGTAAGCC
Suggested primer 2:GAGGTAGCTGAACTCCACCG