Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.190185 |
Chromosome: | chromosome_14 |
Location: | 3704625 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g631900 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTGCATGGGTAAACCGCGTTCCCGATGTA |
Internal bar code: | GTCAGGAGCGCGCGTTCGGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 668 |
LEAP-Seq percent confirming: | 98.5788 |
LEAP-Seq n confirming: | 6867 |
LEAP-Seq n nonconfirming: | 99 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTGTAGAGGAGTGAGGCGG |
Suggested primer 2: | GCGATTCATTATTTTGCCGT |