| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190185 |
| Chromosome: | chromosome 17 |
| Location: | 2718868 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718100 | (1 of 1) PTHR23324:SF56 - CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAGAGGCAACGGCCGATGGAACCCGGC |
| Internal bar code: | TTTCCCCCAGTGTACTGTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 99.3649 |
| LEAP-Seq n confirming: | 11264 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTGATCATGCAATGTTA |
| Suggested primer 2: | CAAGCAGCGATGGAGTAACA |