| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190186 |
| Chromosome: | chromosome 6 |
| Location: | 2050486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264550 | CGL81 | (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997); Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTAAAGCTATCTGTACCAGCGCCCATG |
| Internal bar code: | TGCCGCACCGTTTCCAGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTTTAAACGCAAAGGC |
| Suggested primer 2: | GTGCCGTAAAGTCGAATGGT |